Establishment and Characterization of an Amylase - Producing Human Myeloma Cell
نویسندگان
چکیده
H YPERAMYLASEMIA resulting from ectopic production of amylase by tumor tissue was first described by Weiss et aP in 1 95 1 . Since then, several cases of hyperamylasemia have been reported, especially in pulmonary and ovarian cancers.2’3 However, only one amylase-producing cell line has been established, that being from gastric carcinoma.’4 In addition, malignant plasma cells have also proved to be very difficult to establish, and only a little over ten human myeloma cell lines have been reported. 24 In this study, we established two human myeloma cell lines, named KHM-1A and KHM-1 B, from a patient with IgA A multiple myeloma. KHM-lA cells secreted only IgA, while KHM-1B cells secreted IgA and a-amylase. This is the first report, to the best of our knowledge, of an amylase-producing nonepithelial cell line.
منابع مشابه
Establishment and characterization of an amylase-producing human myeloma cell line.
Two stable lines of IgA lambda-producing plasma cells (KHM-1A and KHM-1B) that were free of the Epstein-Barr virus were established from a patient with multiple myeloma complicated by hyperamylasemia. Surface marker studies of the two cell lines showed that the cells had no surface immunoglobulins but were positive for cytoplasmic immunoglobulins (IgA lambda) and for HLA-DR and PCA-1. Secretion...
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